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Specific examples of different quality metrics based on different classes of data:

 

In vitro/cell-based chemical activity:

Quality Control Metrics: n=>3 data points per concentration; concentration coverage within 3 logs of EC50/IC50; data will include measures of central tendency (mean, median, mode, etc.) and measures of variability (SD, SEM, 95% CI, etc.)

Key Factors for Metadata: Cell line/vector information; assay conditions; instrumentation platform

Data Repositories: PDSP, PubChem, or other suitable repositories

Tracking Identifiers: DOI for weblink

Release Policy: Available immediately after QC metrics met (20% delayed until pre-print deposition in bioRxiv)

 

Mouse pharmacological activity:

Quality Control Metrics: n=>3 data points per dose; attempt at dose response; data will include measures of central tendency (mean, median, mode, etc.) and measures of variability (SD, SEM, 95% CI, etc.)

Key Factors for Metadata: Mouse line RRID#, in-house husbandry conditions, compound vehicle, etc.

Data Repositories: primary data in KOMP; knowledge summary in KMC

Tracking Identifiers: DOI for weblink

Release Policy: Available immediately after QC metrics met, (20% delayed until pre-print deposition in bioRxiv)

 

Mouse protein expression profiling by light-sheet microscopy

Quality Control Metrics: Tracking in at least 2 mice per sex

Key Factors for Metadata: Mouse line RRID#, in-house husbandry conditions, instrumentation platform, analysis software version #

Data Repositories: Primary data in-house, to be made available upon request; knowledge summary in KMC

Tracking Identifiers: DOI for weblink

Release Policy:  Available immediately after QC metrics met (20% delayed until pre-print deposition in bioRxiv)

 

Specific examples of different quality metrics based on different classes of physical resources and reagents:

 

Chemical Resources: Quality control metrics will be adopted from the American Chemical Society, purity >95% by HPLC for distribution and synthetic chemical route.

 

Cell-based Resources: Mycoplasma contamination testing, karyotyping and genomic testing for modifications to detect genetic drift.

 

Mouse Lines: Genotype testing, modification identification, pathogen screening, cryopreservation/cryorecovery and viability.